Figure 3.
Effect of NADPH, BSA, AMP and L-arg on extending S. aureus survival during coculture biofilm growth with P. aeruginosa. Coculture biofilms were grown in vitro on 96-well polystyrene plates for 72 h in static conditions at 37 °C. The different graphs show biofilm cells of P. aeruginosa and S. aureus log10 CFUs/well during biofilm growth in DMEM supplemented with NADPH at 0.2 mM (a), BSA at 5% w/v (b), AMP at 10 mM (c) and L-arg at 0.4% w/v (d) after 12, 24, 36, 48, 60 and 72 h. Conditions were tested in triplicate, and bars represent the mean of three independent experiments. The standard error of the mean is included in the plots. Significant differences between PA14 and Newman CFUs/well at the different incubation times are indicated by asterisks (*p < 0.05; **p < 0.01; ***p < 0.001; and ****p < 0.0001).