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. 2019 Nov 8;9:16304. doi: 10.1038/s41598-019-52671-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Stimulation of monocytes with NETs in vitro. (A) Monocyte subset shift as % of total monoctes, expression of (B) CD11b, (C) CD142 and (D) CX3CR1 of classical, intermediate and non-classical monocytes. Monocyte subsets in percent (%) or mean fluorescence intensity (MFI) levels are displayed. Monocytes were stimulated in whole blood from healthy donors (n = 5) with NETs derived from isolated neutrophils from a healthy donor (n = 1) or vehicle control for 60 minutes. *p < 0.05, **p < 0.01, ***p < 0.001.