Fig. 2.

Resistance to P death is caused by a recessive mutation in fln-2. a Design of Variant Discovery Mapping strategy to select the recessive low P phenotype of N2M in F3 progeny. b Frequency of parental (N2M genotype) reads on chromosome X. For results for all chromosomes see Supplementary Fig. 3. c Normalised frequency of pure parental (N2M genotype) reads on chromosome X. d Illustration of the fln-2(ot611) mutation on FLN-2A, the longest isoform of fln-2. e Necropsy analysis and f lifespan of N2H-derived fln-2(syb202) Y800* strain. N2H survival curve in black, N2M in blue, and fln-2 Y800* in purple. Asterisks indicate p < 0.01, Student’s t test. Error bars represent s.e.m. of three trials. See Supplementary Table 2 for full statistics. See Supplementary data 1 for raw mortality data. Source data are provided as a Source Data file