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. 2019 Nov 8;10:5087. doi: 10.1038/s41467-019-13062-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Reduction of P death by fln-2(ot611) masks effects of high copy number sir-2.1 over-expression (oe) on lifespan. a Lifespan and b necropsy analysis of sir-2.1(oe) and control strains used in previous studies. GA strains are indicated by solid lines: GA468 geIs3 sir-2.1(oe); fln-2(ot611) (blue) and GA707 wuEx166; fln-2(ot611) control (black). LG strains are indicated by dashed lines: LG394 geIs3 sir-2.1(oe) (blue) and LG398 geIs101 control (black). sir-2.1(oe) reduced P death and extended lifespan in LG strains but not GA strains, consistent with both previous, conflicting studies^21,22. Error bar represents s.e.m. of three trials. See Supplementary Table 4 for full statistics. See Supplementary data 1 for raw mortality data. Source data are provided as a Source Data file. c Lifespan and d necropsy analysis of sir-2.1(oe) and control strains after backcrossing four times with N2H. GA outcrossed in solid lines: GA1909 geIs3 sir-2.1(oe) (blue) and GA1907 wuEx166 control (black); LG outcrossed in dashed lines: GA1913 geIs3 sir-2.1(oe) (blue) and GA1915 geIs101 control (black). sir-2.1(oe) strains (blue) from both labs showed a reduced frequency of P death compared to control strains (black) in fln-2(+) background. See Supplementary Table 5 for full statistics. a–d Trials conducted in the presence of 10 μM FUDR. See Supplementary data 1 for raw mortality data. e Lifespan and f necropsy analysis of backcrossed sir-2.1(oe) and control strains, with FUDR excluded. Colour coding as in (c, d). See Supplementary Table 7 for full statistics. See Supplementary data 1 for raw mortality data. g sir-2.1(oe) and 10 μM FUDR interact to protect against progression of bacteria infection. Pharyngeal invasion and widespread infection were scored in worms fed RFP-expressing bacteria. Colour coding as in Fig. 1c. Mean of two trials, n = 16–20 per time point in each trial