Fig. 4.

Effect of daf-12 on daf-2 at 25 °C is partially affected by fln-2(ot611) variation. a Lifespan and b necropsy analysis of daf-2(m41); daf-12(m20) and daf-2(m41) mutants in fln-2(+) or fln-2(ot611) backgrounds. daf-2 fln-2 in green, daf-2 in blue, and daf-2 daf-12 in purple. Asterisks indicate significant differences, p < 0.0001 and 0.01, respectively. Error bars represent s.d. of two trials. See Supplementary Table 10 for full statistics, and Supplementary data 1 for raw mortality data. Source data are provided as a Source Data file. c Lack of lifespan effect by daf-12 in daf-2 p sub-population lifespan in fln-2(+) background. See Supplementary data 1 for raw mortality data