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. 2019 Nov 8;9:16296. doi: 10.1038/s41598-019-52494-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ICH increases RNF34 expression and induces oxidative stress in mouse brain. (A) RNF34 expression in mouse brain injury area was determined by western blotting on 6, 12, 24, and 48 h after ICH surgery. (B) Quantitative analysis of RNF34 expression after normalization against GAPDH. n = 6/group. (C–F) The levels of oxidative stress indicators glutathione (GSH) (C), superoxide dismutase (SOD) (D), malondialdehyde (MDA) (E), and nitric oxide (NO) (F) in brain were examined. n = 6/group. (G–I) RNF34 protein expression was compared with brain levels of GSH (G), SOD (H), MDA (I), and NO (I). n = 30 mice from 5 groups. **P < 0.01 vs. sham.