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. 2019 Nov 8;9:16296. doi: 10.1038/s41598-019-52494-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RNF34 interacts with PGC-1α and promotes ICH-induced PGC-1α ubiquitination in brain tissues. (A–C) The expression of PGC-1α (A), UCP2 (B) and MnSOD (C) were detected with western blotting. **P < 0.01 vs. WT sham; ^##P < 0.01 vs. WT ICH, n = 6/group. (D) Real-time PCR analysis of PGC-1α mRNA expression. (E and F) Western blotting of PGC-1α expression in neurons pretreated with MG132 (10 μg/mL) (E) or chloroquine (10 μg/mL) (F) for 30 min in prior to RNF34 or GFP adenovirus treatment (50 MOI) in the presence of oxyhemoglobin (OxyHb, 10 μmol/L) for 48 h. **P < 0.01 vs. OxyHb + AdGFP; ^##P < 0.01 vs. OxyHb + AdRNF34, n = 6. (G) Brain lysates were immunoprecipitated (IP) with RNF34 antibody and immunoprecipitated proteins were immunoblotted with PGC-1α antibody. (H) Brain lysates from each group were immunoprecipitated with PGC-1α antibody and blotted with ubiquitin antibody. n = 5/group.