Table 1.

Advantages and disadvantages of different NA isolation methods.

Items	ADE-filter	Reported single-tube system30	Column-based kit45	FTA-based assay43	Chip-based assay44
Electrical power	Not needed	Partly; battery-driven centrifugal device is needed	Needed	Partly; battery-driven heater is needed	Partly; battery-driven heater is needed
Pathogen enrichment	Yes	Yes	No	Partly	No
Total time	Under 20 min	60 min	20–30 min for extraction process only	75 min	60 min
Other instruments	Not needed	Centrifugal device needed	Large centrifuge and thermal incubator needed	Not needed	Partly; portable heater
Cost	Low	Low	High	Low	Cost-effective
Sensitivity	100 CFU/mL	100 CFU/mL	102 CFU/mL	3.0 × 100 CFU	3.0 × 100 copies
Specificity	High	High	High	High	High
Reproducibility	High	High	High	High	High
Thermal control	Not needed	Needed	Needed	Not needed	Needed
Surroundings	Household	Laboratory-based	Laboratory-based	Household	Laboratory-based
Complexity of integration with another assay	Easy	Difficult	Impossible	Easy	Easy