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. 2019 Nov 8;9:16298. doi: 10.1038/s41598-019-52801-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Total PV content and relative abundance of β-PV isoforms in Gadus morhua and Scomber scombrus muscles. (a) Typical Coomasie Blue-stained SDS-PAGE gel of (C1, C2) Gadus morhua and (M1,M2) Scomber scombrus muscle extracts and the PV content estimated from monomer band quantification. The protein load per lane was 5 μg for the extracts and 0.5 μg for gmPV1, which was used as a control. Numbers on the right side indicate the molecular weights of markers in kDa. (b) SDS-PAGE analysis of the intrinsic proteolysis and solubility of PV in muscle extracts. Freshly prepared extracts were (4) stored at 4 °C, (37) heated for 15 min at 37 °C, cooled at 4 °C, and separated into soluble (S4) and insoluble (P4) fractions by ultracentrifugation. Numbers on the right side indicate the molecular weights of markers in kDa. (c) Mr determination for each of the different β-PV isoforms isolated from muscle extracts by FTICR-MS, considering the processing of M1 and the acetylation of A2^39,52. The original gels of panels a and b are displayed in supplementary Fig. S1.