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. 2019 Nov 8;9:16298. doi: 10.1038/s41598-019-52801-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunoreactivity of the denatured state of β-PV: linear IgE epitopes. (a) Coomassie blue-stained SDS-PAGE gel of β-PV isoforms. (b) Western blots of β-PV isoforms probed with PARV19. The relative loading amount is depicted at the top. (c) Western blots of β-PV isoforms probed with different fish-allergic patient sera for IgE binding. Displayed blots correspond to membranes developed independently. Numbers on the right side of gels and membranes in panels a and b correspond to the molecular weights of markers in kDa. Full-length gels and blots are displayed in supplementary Figs 2S and 3S.