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. 2019 Nov 8;9:16298. doi: 10.1038/s41598-019-52801-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Conformational properties of the Ca²⁺-bound globular folds of the distinct β-PV isoforms. (a) DLS analysis of the association state and hydrodynamic features. (b) Secondary structure probed by far-UV CD spectra. Spectra were recorded before (circles) and after heating (triangles) at 95 °C. (c) Thermal denaturation monitored changes in θ₂₂₂ as a function of temperature. Symbol key: •, gmPV1; ◦, gmPV2; , sjPV1; , sjPV2. (d) SDS-PAGE analysis of proteinase K and pepsin digestion products of β-PV isoforms. Digestions were performed using a 1/50 protease/β-PV ratio. The full-length gel is displayed in supplementary Fig. 4S.

Inline graphic — Conformational properties of the Ca²⁺-bound globular folds of the distinct β-PV isoforms. (a) DLS analysis of the association state and hydrodynamic features. (b) Secondary structure probed by far-UV CD spectra. Spectra were recorded before (circles) and after heating (triangles) at 95 °C. (c) Thermal denaturation monitored changes in θ₂₂₂ as a function of temperature. Symbol key: •, gmPV1; ◦, gmPV2; , sjPV1; , sjPV2. (d) SDS-PAGE analysis of proteinase K and pepsin digestion products of β-PV isoforms. Digestions were performed using a 1/50 protease/β-PV ratio. The full-length gel is displayed in supplementary Fig. 4S.