Skip to main content
. 2019 Nov 8;9:16348. doi: 10.1038/s41598-019-52626-3

Figure 6.

Figure 6

Naringenin treatment was effective even after the infection establishment. The A549 cells were infected with ZV BR 2015/15261 (MOI 0.1) and treated (one time) with NAR (125 μM) or IFN-α 2A (200 IU/mL) at different times points after the infection establishment, at 0, 1, 2, 4, 6, and 24 hours post-infection (hpi). After 48 h of infection, the A549 cells were harvested and stained with mouse anti-flavivirus E protein (4G2) monoclonal antibody for the flow cytometry analyses. (A) Schematic representation of the experimental design. (B) Representative density plot showing the frequency of A549-infected cells (4G2+). (C) Frequency of ZIKV-infected A549 cells (4G2+). (D) The viral titers detected by foci-forming immunodetection assay (FFUC6/36/mL) in the supernatant of A549-infected cells. The dotted line represents A549 cells treated with NAR, and the continuous line represents A549 treated with recombinant IFN-α 2A. The data represent three independent experiments for which each was performed in technical triplicate then analyzed by one-way ANOVA followed by Tukey´s Multiple Comparison Test (*p < 0.05 vs ZIKV-infected and untreated cells).