Table 2.

ISSR primers and their annealing temperatures

Sl no.	ISSR primers	Annealing temperature (°C)
1.	(GTGC)4	61.9
2.	(GACAC)4	58.3
3.	(CT)8G	46.8
4.	(AG)8	44.7
5.	(GTG)5	53.1
6.	(GGA)4	42.8
7.	(GAC)5	52.6
8.	(GACA)4	47.4
9.	(GA)9T	48.8
10.	(CTGC)4	60.4
11.	(GA)8G	46.5
12.	A(CT)8	47.0
13.	(GCT)8	69.5
14.	(AGG)6	57.5
15.	T(GA)9	50.2
16.	(CT)8A	44.7
17.	G(CT)8	48.8
18.	(CCA)5	54.8
19.	(CGA)4	46.5
20.	(GACA)4G	49.0

A Adinine; G Guanine; C Cytosine; T Thyamine