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. 2019 Nov 8;9:16322. doi: 10.1038/s41598-019-52892-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The optimal dose of topical BDP in combination with topical Cal in mice with IMQ-induced psoriasis-like dermatitis. Mice received the topical application of IMQ cream on the ear for 6 consecutive days and were treated with 0.6 nmol of Cal, 0.0006 (A), 0.006 (B), and 0.06 nmol (C) of BDP or the combination of Cal and BDP 1 h before IMQ application. Representative gross appearances of skin lesions one day after the final IMQ application (D). Normal mice were used as a negative control. Ear thickness was measured every day. The values and vertical bar represent the mean ± SE of 4 mice. ***p < 0.001 vs. vehicle group, ⁺p < 0.05, ⁺⁺⁺p < 0.001 vs. Cal group, ^###p < 0.001 vs. BDP group by Tukey test among the vehicle, Cal, BDP, and Cal/BDP groups.