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. 2019 Nov 8;9:16322. doi: 10.1038/s41598-019-52892-1

Figure 3.

Figure 3

Synergistic effects of the topical combination of Cal and BDP on IMQ-induced psoriasis-like dermatitis in mice through suppressing the expression of cytokines related to the IL-23/T17 pathogenic axis. Mice received the topical application of IMQ cream on the ear for 6 consecutive days and were treated with 0.6 nmol Cal, 0.06 nmol BDP, or the combination of 0.6 nmol Cal and 0.06 nmol BDP 1 h before each IMQ application. (A) Effects of Cal, BDP, and Cal/BDP on ear thickness. Ear thickness was measured every day. The values and vertical bar represent the mean ± SE of 6 mice. ***p < 0.001 vs. vehicle group, +++p < 0.001 vs. Cal group, ###p < 0.001 vs. BDP group by Tukey test among the vehicle, Cal, BDP, and Cal/BDP groups. (B) Inhibition of the expression of cytokines related to the IL-23/T17 pathogenic axis in IMQ-induced skin lesion. The expression in IMQ-induced skin lesions of the indicated mRNAs, which are related to the IL-23/T17 pathogenic axis, was analyzed by quantitative real-time RT-PCR. The expression is relative to the housekeeping gene Gapdh. Each symbol represents an individual mouse, and the horizontal lines indicate the means of the groups (n = 6). Similar results were obtained in two independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 by Tukey test among the vehicle, Cal, BDP, and Cal/BDP groups.