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. 2019 Nov 8;9:16307. doi: 10.1038/s41598-019-52592-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A scheme of the swimming assay. (a) The whole view of the swimmill equipment used in this study. (b) The size of the cylinder chambers. (c) The enlarged view of the swimming chamber. Zebrafish swim in the water flow to stay at the same position. Two plastic meshes were put in the small cylinder to avoid the fatigued fish hit to the spinning propeller. M: mesh; P: propeller; C: high speed camera; Blue arrow: water flow. (d) Protocol of incremental changes in water velocity.