Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 8;9:16350. doi: 10.1038/s41598-019-52424-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Annotation of the MinION assembly of Escherichia coli. (a) The E. coli O157:H7 chromosome was sequenced and assembled into a final consensus of 5,482,542 nucleotides. The annotation of the genome provided the location of 5,748 coding sequences (CDS), 106 tRNAs, 29 rRNAs, 6 regulatory regions, and 1 repeat regions. For imaging purposes, only the 6 regulatory regions (green), the one repeat region (brown) and the CDS of two virulence factors (yellow) are shown magnified. The LEE (locus of enterocyte effacement) is highlighted at position 4,603,699 to 4,636,299, and the Shiga Toxin subunits are shown at position 3,181,004 to 3,180,992 for demonstration purposes. (b) The E. coli pO157 plasmid was sequenced and assembled into a final consensus of 94,503 nucleotides. The annotation shows all 124 coding sequences (CDS) in yellow. The CDS of three well-known virulence factors are highlighted: hemolysin (ehx) at position 16,584 to 19,578, catalase-peroxidase (katP) at position 76,704 to 78,356, and the type II secretion system (T2SS) at position 64,056 to 85,694 for demonstration purposes.