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. 2019 Nov 8;9:16379. doi: 10.1038/s41598-019-52852-9

Figure 6.

Figure 6

Effect of HSA levels on ruxolitinib-mediated changes of JAK2V617F signaling and cyclin expression levels. JAK2wt K562 and JAK2V617F+ HEL and SET-2 cells were cultured with FBS or 80 μM HSA, or 8 μM HSA, or medium alone (0 μM HSA). Cells were left untreated or treated with the indicated amount of ruxolitinib (Rxn). Change in the protein levels of inactive and phosphorylated STAT5 and JAK2 were measured after 3 hours of ruxolitinib treatment, whereas cyclin p27Kip, cyclin D3 and GAPDH protein levels were detected after 24 hours. Immunoblot analysis was performed as described in the method section.