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. 2019 Nov 8;9:16378. doi: 10.1038/s41598-019-52816-z

Figure 1.

Figure 1

Development of VIM promoter–driven RFP expression system using CRC cell lines. (a) Structure for VIM promoter–driven RFP expression vector without or with VIM 3′-UTR, VR or VRV3 vector, respectively. Photographs of HCT116 cells stably transfected with VR and VRV3 vectors, HCT116-VR and HCT116-VRV3. Scale bars: 50 μm. MCS, multi-cloning site. (b) Photographs of HCT116-VRV3 and RKO-VRV3 cells treated without or with TNF-α (20 ng/ml), IL-1β (1 ng/ml), TGF-β (10 ng/ml), HGF (50 ng/ml), IGF-1 (20 ng/ml), EGF (20 ng/ml), or bFGF (10 ng/ml) for 48 h. Scale bars: 50 μm. (c) expression of RFP in HCT116-VRV3 and RKO-VRV3 cells treated without or with TNF-α (20 ng/ml), IL-1β (1 ng/ml), TGF-β (10 ng/ml), HGF (50 ng/ml), IGF-1 (20 ng/ml), EGF (20 ng/ml), or bFGF (10 ng/ml) for 48 h. β-actin was used as a loading control.