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. 2019 Nov 8;9:16378. doi: 10.1038/s41598-019-52816-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reversibility of TNF-α– and IL-1β–induced RFP expression and EMT phenotype. (a) Photographs of HCT116-VRV3 and RKO-VRV3 cells after removal of cytokines following treatment with TNF-α (20 ng/ml) or IL-1β (1 ng/ml) for 48 h. Scale bars: 50 μm. (b) Expression of RFP, epithelial markers (CDH1 and CK20), and a mesenchymal marker (α-SMA) in HCT116-VRV3 and RKO-VRV3 cells after removal of cytokines following treatment with TNF-α (20 ng/ml) or IL-1β (1 ng/ml) for 48 h. β-actin was used as a loading control.