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. 2019 Nov 8;7(21):e14274. doi: 10.14814/phy2.14274

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© 2019 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Electrogenic short‐circuit current (I _sc) induced by DL‐Met. Increasing concentrations of DL‐Met in Na⁺ media induced changes in I _sc (A) in pyloric caeca (PC), (B) midgut (MG), and (C) hindgut (HG) of triploid (● 3N) and diploid (■ 2N) with segmental representative traces (D, E, and F) presented on the right side. The DL Met‐induced current was plotted as a function of the extracellular substrate concentrations using Michaelis–Menten equation. Experiments were carried out with DL‐Met gradient from 0 to 150 µmol/L (21 increasing sequential concentration). Each data point was expressed as mean ± SEM (n = 13–18).