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. 2019 Nov 8;9:16331. doi: 10.1038/s41598-019-52743-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GFP-Sktl and PI(4,5)P₂ translocated to the rear side of epidermal cells during wound healing. (a,b) Localization of GFP-Sktl fusion protein, shown in green, after injury. (a) 0 h after injury. (b) 7 h after injury. (c,d’) Distribution of PI(4,5)P2, analysed using PH_PLCδ-Cerulean in unwounded (c,c’) or wounded epidermis 7 h after injury (d,d’). (c,d) Epifluorescence of Cerulean from PH_PLCδ-Cerulean reporter in green. (c’,d’) Plotting of the fluorescence intensities along the frontal-rear axis of the cell in regard to the wound, marked with a white line with two arrowheads each in (c,d). A58-GAL4 is a larval epidermis-specific driver. A58-GAL4 UAS-GFP-sktl was abbreviated as A58 > GFP-sktl. Cell boundaries were stained with anti-FasIII antibody in red. The asterisk marks the wound hole and the white dotted line indicates the wound margin. Scale bars: 50 μm. (e) Rear localization index values for PH_PLCδ-Cerulean were calculated (see Methods). Mann–Whitney U-test was used for statistical significance.