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. 2019 Nov 8;9:16331. doi: 10.1038/s41598-019-52743-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sktl localization required JNK and integrins, but not PVR. Sktl localization was analysed in the larval epidermis expressing GFP-Sktl, shown in white, 7 h after wounding. (a,d) Controls. (b) A58 > bsk^DN. (c) Quantification of the rear localization of GFP-Sktl in the first two rows of cells, presented with the rear localization index value (see Methods for calculation). (e) A58 > βPS-RNAi. (A58 > βPS-i, hereafter). (f) A58 > talin-i. (g) A58 > PVR^DN. The asterisk indicates the wound hole. (a’,b’,d’–g’) Plotting of the GFP fluorescence intensities along the frontal-rear axis of the cells in the 1st row (black) and the 2nd row (grey) from the wound margin. The axes were marked in white lines with two arrowheads in (a,b,d–g). Scale bar: 50 μm. For statistical analysis, Mann–Whitney U-test was used.