Figure 2:

Expression of p-α-syn, GFAP and IBA1 in the mPFC of 7-month old wt and A53T mice. Representative IF microphotographs of the DAPI, p-α-syn, GFAP, IBA1 and the merged image in 7 mo wt mice (A-C) and A53T mice (D-F) used for p-α-syn, GFAP and IBA1 densitometry and IBA1 call density analysis. Image J was used to quantify the intensity of p-α-syn, GFAP and IBA1 staining and density of IBA1 positive cells. Increased expression of the p-α-Syn (G), GFAP (H) and IBA1 (I) was observed in A53T mice compared to wt mice. The A53T mice showed increased density of IBA1 positive cells (J). (Student’s t-test, n=5/group; *p<0.05, **p<0.01)