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Quantification of inhibitory pre-synaptic terminals in 7-month old wt and A53T mice. Representative high magnification IF microphotographs of the GAD65 and synaptophysin and merged images of the mPFC of 7-mo wt mice (A) and A53T mice (B). Synaptophysin in green and GAD 65 in red. Immunocytochemistry-based assay showed reduced number of colocalized GAD65/synaptophysin pre-synaptic terminals in 7 mo A53T mice compared to wt littermates. (Student’s t-test, n=3/group; *p<0.05)
