Fig. 4.

Agarose gel electrophoresis for the amplification and genetic diversity analysis of MHC-DQA gene. The blood samples were collected from the experimentally infested rabbits at week 4 post-infestation for amplification of MHC class II DQA gene by direct PCR, and the genetic diversity on the second exon of MHC-DQA gene between two breeds of rabbit were analyzed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method with Mbo II enzyme. a Amplification of MHC-DQA gene in QiXing rabbits and IRA groups. Lane M: DL2000 DNA marker; Lanes 1–18: amplicons from experimentally infested rabbits in each group. b Analysis of genetic diversity in MHC-DQA gene by PCR-RFLP method with Mbo II enzyme. Lane M: D50 DNA marker; Lanes 1–18: amplicons from the experimentally infested rabbits in each group. Abbreviations: QX, the QiXing rabbit propagated by the Sichuan Animal Sciences Academy, China; IRA, the IRA rabbit