Fig. 2.

WZ35 inhibits MDA-MB-231 xenograft tumor growth and metastasis in vivo. a A growth curve analysis of the tumor growth in curcumin, WZ35 or Castor oil treated groups. b Expression of ki67 in tumor tissues was determined by immunohistochemistry. c Mice body weight was measured during the 17-day treatment of WZ35 and curcumin. d Representative microscopic images of lung metastatic lesions at 21 days after treatment of mice with curcumin or WZ35 (0.2 mL, 25 mg/kg for each). e The number of lung metastatic tumors was calculated. f Immunofluorescence were used to test the expression level of E-cadherin, N-cadherin, MMP-2 and MMP-9 from curcumin, WZ35, Vehicle and control group. Data are presented as mean ± SD, ***p < 0.001