Fig. 1.

CircAGFG1 was overexpressed in cervical cancer and its downregulation restrained the proliferation and migration. a CircAGFG1 expression in cervical cancer cells (HeLa, C-33a, SiHa and HCC94) and normal cervical cell End1/E6E7 was measured by qRT-PCR. b Interference efficiency of three siRNAs containing si/circAGFG1#1/2/3 in HeLa and SiHa cells via qRT-PCR. CircAGFG1-silenced HeLa and SiHa cells were used for following mechanism assays. 0.1–1 pmol/150–300 ng of siRNA was used. c, d CCK-8 and EdU assays were performed in HeLa and SiHa cells to detect cell viability at 0, 24, 48, 72 and 96 h and cell proliferation. e Cell apoptosis was measured via flow cytometry analysis in HeLa and SiHa cells. f Transwell assay for cell migration ability in HeLa and SiHa cells. Data of three experimental results were exhibited as the mean ± standard deviation (SD). ^*P < 0.05, ^**P < 0.01