Fig. 2.

MiR-370-3p was downregulated in cervical cancer and negatively modulated by circAGFG1. a RNA-FISH images exhibiting the subcellular locations of circAGFG1 and miR-370-3p in HeLa and SiHa cells. The quantification results were shown on the right. b Influence of circAGFG1 knockdown using two siRNAs on miR-370-3p levels in HeLa and SiHa cells via qRT-PCR. c Low expression of miR-370-3p in cervical cancer cells was revealed via qRT-PCR, in comparison with End1/E6E7 cells. d The potential wild-type and mutated binding sites of circAGFG1 for miR-370-3p. e, f Overexpression efficiency of miR-370-3p mimic was determined by qRT-PCR, followed by luciferase reporter activity affirming the binding between circAGFG1 and miR-370-3p. The specific interaction between circAGFG1 and miR-370-3p compared with other circRNAs was also affirmed. These assays were implemented in HeLa and SiHa cells. (G-H) RNA pull down and RIP assays followed by qRT-PCR were conducted in HeLa and SiHa cells for the combination of circAGFG1 with miR-370-3p. Data of three experimental results were exhibited as the mean ± standard deviation (SD). ^*P < 0.05, ^**P < 0.01