Fig. 3.

CircAGFG1 activated RAF/MEK/ERK pathway by sponging miR-370-3p. a The miR-370-3p binding site on RAF1 (WT) and mutant binding site. Additionally, the sequence of miR-370-5p was also displayed. b qRT-PCR detection of overexpression efficiency of pcDNA3.1/circAGFG1, which was carried out in HeLa and SiHa cells. c Luciferase reporter assay to elucidate the regulation of circAGFG1 and miR-370-3p on RAF1 in HeLa and SiHa cells. d In HeLa and SiHa cells, RIP and qRT-PCR experiments were utilized to validate the interplay between miR-370-3p and RAF1. e Interference efficiency of miR-370-3p inhibitor in HeLa and SiHa cells. f Impact of circAGFG1 and miR-370-3p on RAF1 expression at mRNA level, as estimated by qRT-PCR in HeLa and SiHa cells. g Impacts of circAGFG1 and miR-370-3p on the protein levels of RAF1 as well as RAF/MEK/ERK pathway markers containing p-RAF1, p-MEK1/2 and p-ERK1/2 in both HeLa and SiHa cells. Data of three experimental results were exhibited as the mean ± standard deviation (SD). ^*P < 0.05, ^**P < 0.01