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. 2019 Nov 8;19:1075. doi: 10.1186/s12885-019-6312-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Establishment of radioresistant HCC cells. a A representative image of colony formation in parental and their radioresistant HCC cells treated with various dose of IR (0.0, 4.0 and 6.0 Gy) after 14 days. b The results of colony formation. c Survival fractions of parental and their radioresistant HCC cells were obtained from the results of the colony-forming assays. d Flow cytometric detection of apoptosis in parental and their radioresistant HCC cells treated with various dose of IR (0.0, 4.0 and 6.0 Gy). Data represent the mean ± S.E. of three individual experiments with triplicates. *p < 0.05 and**p < 0.01