Fig. 2.

Effect of Aurora-A knockdown on in vitro radiosensitivity of radioresistant HCC cells. a RT-PCR and Western blot was used to detect the Aurora-A mRNA and protein expression in radioresistant and their parental HCC cells. The internal control was GAPDH. b RT-PCR and Western blot was used to detect the Aurora-A mRNA and protein expression in the stably transfected HCC cells (HepG2-R/shAuro or HepG2-R/shcontrol). The internal control was GAPDH. c A representative image of colony formation in the stably tansfected HCC cells treated with various dose of IR (0.0 and 4.0 Gy) after 14 days. d Survival fractions of the stably transfected HCC cells were obtained from the results of the MTT assays. e Flow cytometric detection of apoptosis in the stably transfected HCC cells treated with various dose of IR (0.0 and 4.0Gy). Data represent the mean ± S.E. of three individual experiments with triplicates. *p < 0.05 and**p < 0.01