Fig. 1.

Mitochondria exchange between DNA–damaged and healthy fibroblasts. A Experimental setup of co–cultures. IR, ionizing radiation (x–rays). b Representative images of mitochondria exchange between unirradiated (MitoT Deep Red) and 6–Gy irradiated (MitoT Green) fibroblasts (arrow: cell–cell contact; white boxed area: Super–resolution radial fluctuation (SRRF) images). c Pearson correlation analysis of colocalization between MitoT Deep Red and Green in acceptor cells at 24–h of co–culture under indicated pretreatments. n = 30–50 cells. d Representative shape description images of aspect ratio (AR) using single cell analysis of acceptor cells at 2–h of co–culture as in b. Average AR value of MitoT Green = 1.67 (used as control for e), maximum AR = 3.7. e Comparison of indicated conditions to 2–h in acceptor of MitoT Green. f Size distribution of AR und indicated conditions. Data represent mean ± SD (two–sided t–test; ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.005)