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. 2019 Nov 8;15:402. doi: 10.1186/s12917-019-2074-7

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Determination of cytokine responses in splenocyte of immunized mice with different recombinant proteins. Levels of each cytokine were quantified (pg/mL) by ELISA. Mean comparison of treatments were carried out employing Tukey’s test with significance level of 0.05. Each value represents the mean of triplicates ± SD of cytokine responses from five samples. The experiment was replicated three times. Different letters indicate significantly different between experimental groups (P < .05)