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. 2019 Nov 5;12:8935–8945. doi: 10.2147/OTT.S210739

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© 2019 Xue et al.

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The inhibitory effect of miR-139-3p on SK-OV-3 cell aggressiveness was rescued by the reexpression of ELAVL1. (A) qRT-PCR analysis of ELAVL1 mRNA levels in SK-OV-3 cells co-transfected with ELAVL1 plasmid in combination with either miR-139-3p mimic or miR-NC. GAPDH was used as an internal control. (B) Western blot analysis of ELAVL1 protein levels in SK-OV-3 cells co-transfected in combination with either miR-139-3p mimic or miR-NC. (C) CCK8 assay showing cell proliferation in SK-OV-3 cells co-transfected with ELAVL1 plasmid in combination with either miR-139-3p mimic or miR-NC. (D) Estimation of number of colonies in SK-OV-3 cells stable expressing miR-139-3p or miR-NC transfected with ELAVL1 plasmid without 3ʹ-UTR. (E) Wound-healing assay estimating migration of SK-OV-3 cells co-transfected with ELAVL1 plasmid in combination with either miR-139-3p mimic or miR-NC. (F) Transwell assay estimating the invasiveness of SK-OV-3 cells co-transfected with ELAVL1 plasmid in combination with either miR-139-3p mimic or miR-NC. **P<0.01 compared to miR-NC, ^##P<0.01 compared to miR-139-3p.