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. 2019 Nov 5;11:9353–9369. doi: 10.2147/CMAR.S219689

Figure 6.

Figure 6

miR-124-3p downregulation contributes to MST4 upregulation in GC. (A) Three microRNAs that target MST4 were screened according to a microRNA database TargetScan (www.targetscan.org). (B) qRT-PCR was used to determine the expression level of three miRNAs in GCTs and corresponding ANGMs. (C) Data of luciferase reporter assay. Relative luciferase activity was analyzed after MST4 wildtype or mutant reporter plasmids were co-transfected into 293T cells infected with miR-124-3p lentivirus or control lentivirus. (D) Western blot showed MST4 expression in AGS cells with miR-124-3p overexpression and MKN45 cells with miR-124-3p knockdown. (E) IHC assays showed miR-124-3p expression was negatively associated with MST4 expression in GC. Representative IHC pictures about MST4 expression in GCTs with high miR-124-3p expression and low miR-124-3p expression according to the median miR-124-3p expression level by qRT-PCR determined in (B). **P < 0.01; ***P < 0.001.