Fig. 2.

MiR-212/132 are upregulated following hypoxia in human brain microvascular endothelial cells, brain capillaries, and serum exosomes. MiR-212/132 levels were measured in oxygen-glucose deprivation (OGD)- or OGD/re-oxygenation (OGD/reox)-treated human brain microvascular endothelial hCMEC/D3 cell line (a) by qPCR. Data were normalized to control cells cultured in standard conditions (normox) which was set as 1 and is presented as an average of three independent experiments with standard deviations. b MiR-212/132 levels were tested by qPCR in human brain capillaries isolated post mortem from individuals who died from traumatic brain injury (TBI) and normalized to expression levels in a non-injured brain region (n = 5). c MiR-212/132 levels were estimated by qPCR in exosomes isolated from patients who suffered from malignant hemispheric stroke. MiR levels measured in samples collected at day 3 post stroke were normalized to levels at day 7 post stroke. Values are presented as mean with standard deviation (n = 14 per group), *p < 0.05, ***p < 0.001