Fig. 3.

MiR-212/132 can be detected in brain capillaries and their over-expression leads to decrease in barrier properties of endothelial monolayer. MiR-212/132 were detected by in situ hybridization using a specific miR-132 and miR-212 probe. CD31, a marker for endothelial cells, was detected by immunofluorescence (a). CEND cells transfected with control (scr) or pre-miR-212/132 (miR-212/132) showed decreased barrier properties as measured by transendothelial electrical resistance (TEER) (b) and permeability of fluorescein (332 Da), 4 kDa and 10 kDa FITC-Dextran (c). d The migration rate of pre-miR-212/132 transfected cEND was measured in the wound healing assay. Images of the migration area were captured 0 and 24 h after removal of the separating chamber. Cell-free area was quantified and presented in arbitrary units (AU). Data are presented as an average of three independent experiments with standard deviations, *p < 0.05, **p < 0.01