Fig. 5.

Effects of over-expression of miR-212/132 precursors (pre-) and inhibitors (anti-) on mRNA and protein expression of target genes in cEND cells as compared to the effects of scrambled (scr) controls. CEND were transfected with pre-miR-212/132 or anti-miR-212/132. CEND transfected with anti-miR-212/132 were subjected to 4-h OGD. Analysis of mRNA expression of claudin-1 (Cldn1) (a), junctional adhesion molecule 3 (Jam3) (b), occludin (Ocln) (c), and tight junction-associated protein 1 (Tjap1) (d) was performed by qPCR. e Protein levels of Claudin-1, Jam-C, Ocln, and Tjap1 in normoxic cEND (normox) over-expressing pre-miR-212/132 and in hypoxic cEND (OGD) transfected with anti-miR-212/132. Representative images were chosen. Numbers under the respective bands show protein levels normalized to β-actin and to scrambled (scr) control. Data are presented as an average of three independent experiments with standard deviations, *p < 0.05