Fig. 3.

PCR amplification of 16S rRNA of metagenomic DNA isolated from stool, fish gut, soil and milk samples. The PCR products were resolved on a 1.2% agarose gel, stained with ethidium bromide and photographed in a gel documentation system. Lane M: 100 bp DNA Ladder RTU (GenedireX); lanes 1–4; 16S rRNA gene PCR amplification of DNA isolated by method A—soil, fish gut (silver carp), fish gut (T. putitora), stool, respectively; lanes 5–8; 16S rRNA gene PCR amplification of DNA isolated by method B- soil, fish gut (silver carp), fish gut (T. putitora), stool, respectively; lanes 9–12: 16S rRNA gene PCR amplification of DNA isolated by method C—soil, stool, fish gut (silver carp), fish gut (T. putitora), respectively; lanes 13–15: 16S rRNA gene PCR amplification of milk DNA, isolated by method A, modified method B, modified method C