Table 1.
Concentration of metagenomic DNA isolated from different human and environmental samples
| S. no. | Sample | Methods | Concentration in ng/µl (mean ± SD) | 260/280 (mean ± SD) | 260/230 (mean ± SD) |
|---|---|---|---|---|---|
| Method_A | |||||
| 1 | Stool | 141.9 ± 35.6 | 1.775 ± 0.06 | 1.285 ± 0.07 | |
| 2 | Soil | 430.85 ± 82.80 | 1.665 ± 0.04 | 1.24 ± 0.16 | |
| 3 | Fish-gut (silver carp) | 238.6 ± 64.48 | 1.7 ± 0.07 | 1.37 ± 0.07 | |
| 4 | Fish-gut (T. putitora) | 92.1 ± 4.10 | 1.475 ± 0.50 | 1.15 ± 0.37 | |
| 5 | Milk | 104.1 ± 36.48 | 1.72 ± 0.33 | 1.215 ± 0.10 | |
| Method_B | |||||
| 1 | Stool | 30.75 ± 7.99 | 1.905 ± 0.09 | 0.63 ± 0 | |
| 2 | Soil | 561.3 ± 65.19 | 1.915 ± 0.07 | 1.55 ± 0.19 | |
| 3 | Fish-gut (silver carp) | 271 ± 32.80 | 1.98 ± 0.09 | 1.11 ± 0.29 | |
| 4 | Fish-gut (T. putitora) | 72.2 ± 23.75 | 1.42 ± 0.11 | 0.875 ± 0.13 | |
| 5 | Milk | 168.095 ± 16.83 | 1.73 ± 0.06 | 1.31 ± 0.00 | |
| Method_C | |||||
| 1 | Stool | 964.95 ± 60.8 | 2.145 ± 0.02 | 1.89 ± 0.12 | |
| 2 | Soil | 76.35 ± 20.7 | 1.66 ± 0.22 | 0.97 ± 0.00 | |
| 3 | Fish-gut (silver carp) | 186.85 ± 6.15 | 2.025 ± 0.23 | 1.41 ± 0.38 | |
| 4 | Fish-gut (T. putitora) | 56.6 ± 2.12 | 1.5 ± 0 | 0.98 ± 0.10 | |
| 5 | Milk | 20.35 ± 7.51 | 1.60 ± 0.18 | 0.6 ± 0.46 |
sd standard deviation