Fig. 1.

Comparison of Ag43 steady-state levels in WT and osmY-null strains.

A. WT and ΔosmY cells were assayed for quantitative proteomics using LC-MS/MS; shown are normalized Ag43 α- and β-domain spectral counts.

B. WT and ΔosmY cell pellets were resuspended in SDS-reducing sample buffer. After boiling at 95°C for 5 min or not, equal volumes were analyzed by SDS-PAGE and western blot using antiserum raised against the indicated proteins.

C. Autoaggregation of WT and ΔosmY cells was assayed by taking samples 1 cm below the liquid surface and measuring optical density at 600 nm.

D. Graph shows the quantitative RT-PCR analysis of Ag43 α- and β-domain mRNA levels in ΔosmY relative to WT.