Fig. 4.

Cardiac Akt, mammalian target of rapamycin, p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase 1/2 (ERK1/2) signalling in 3-month-old offspring. a Representative bands from one western blot for each protein analysed. b, c Mean ± SEM relative abundance of total and phosphorylated forms of Akt, S6 ribosomal protein, 4EBP1, p38 MAPK and ERK1/2 proteins in the heart of 3-month-old male (n = 10–11, b) and female (n = 7–8, c) offspring of control, phosphate-buffered saline (PBS)-infused pregnant mice (C/PBS) and obese pregnant mice infused either with PBS (Ob/PBS) or adiponectin (Ob/AND) from E14.5 to 18.5. Values in bold indicate significant overall effect of maternal treatment group by one-way analysis of variance. Values with different superscripts, a or b, are significantly different from one another by pairwise post hoc comparison (P < 0.05, Holm–Sidak test)