. 2019 May 10;44(2):488–499. doi: 10.1038/s41366-019-0374-4

Table 2.

Cardiac fibrosis in 3- and 6-month-old offspring

	Con/PBS	Ob/PBS	Ob/ADN	P(ANOVA)
Male offspring
3-month collagen expression	n = 10	n = 14	n = 14
Col1a1	1.00 ± 0.34	1.44 ± 0.34	1.56 ± 0.33	0.49
Col3a1	1.00 ± 0.23	1.52 ± 0.28	1.27 ± 0.30	0.76^c
6-month collagen expression	n = 10	n = 10	n = 9
Col1a1	1.00 ± 0.15	1.65 ± 0.39	0.91 ± 0.14	0.12
Col3a1	1.00 ± 0.17	1.67 ± 0.40	0.82 ± 0.13	0.08
Collagen content (picrosirius)	n = 6	n = 5	n = 10
% cross-sectional area	5.2 ± 1.2	4.3 ± 1.4	2.9 ± 0.6	0.22
Female offspring
3-month collagen expression	n = 7	n = 6	n = 10
Col1a1	1.00 ± 0.19	0.79 ± 0.19	1.68 ± 0.34	0.07
Col3a1	1.00 ± 0.08^a	1.16 ± 0.18^a	2.10 ± 0.32^b	<0.01
6-month collagen expression	n = 10	n = 10	n = 8
Col1a1	1.00 ± 0.08^a	1.43 ± 0.25^a	2.63 ± 0.55^b	<0.01^c
Col3a1	1.00 ± 0.10^a	1.38 ± 0.23^ab	1.87 ± 0.30^b	0.03
Collagen content (picrosirius)	n = 8	n = 9	n = 7
% cross-sectional area	2.6 ± 0.3^a	3.7 ± 0.8^b	6.3 ± 0.8^b	<0.01

Mean ± SEM relative expression of Col1a1 and Col3a1 and collagen content determined by picrosirius staining in 3- and 6-month-old offspring of control, PBS-infused pregnant mice and obese pregnant mice infused either with PBS or adiponectin from E14.5 to 18.5. Values in bold indicate significant overall effect of maternal treatment group by one-way ANOVA. Values with different superscripts, a or b, are significantly different from one another by pairwise post hoc comparison (P < 0.05, Holm–Sidak test)

ADN adiponectin, ANOVA analysis of variance, Con control, Ob obese, PBS phosphate-buffered saline

^cIndicates ANOVA performed on log transformed data. Statistical analysis of collagen content performed on combined 3- and 6-month old offspring