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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Am J Ophthalmol. 2019 May 10;206:113–131. doi: 10.1016/j.ajo.2019.04.033

Figure 1. Gene expression of FGF ligands in early optic vesicles (OVs) and forebrain neurospheres (FBNs) derived from hiPSCs.

Figure 1.

A) Schematic showing the timeline of early hiPSC differentiation to OVs and FBNs. At day 0 (D0), pluripotent hiPSCs are subjected to a well-established, fully defined differentiation protocol that generates anterior neuroepithelium/eye field cells by D10, followed 10 days later by the appearance of OVs and FBNs74, 75, 78. B) hiPSC-OVs and hiPSC-FBNs can be easily distinguished in live cultures by their light microscopic appearances, manually picked, and cultured separately. C) Comparative microarray analysis showing the relative expression (see accompanying heat map legend) of FGF ligands in wildtype hiPSC-OVs vs. hiPSC-FBNs isolated from the same cultures at D2074. D) Quantitative real-time PCR analysis at D30 revealed a significantly sustained increase in the expression of FGF9 and FGF19 in hiPSC-OVs relative to hiPSC-FBNs, but not FGF8 (**P<0.01).