KEY RESOURCES TABLE
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Brachyury (goat polyclonal) | R&D Systems, Inc., Minneapolis, MN | AF2085 |
| Carbonic anhydrase 3 (goat polyclonal) | Santa Cruz Biotechnology, Inc., Dallas, TX | sc-50715 |
| Vimentin (rabbit monoclonal) | Cell Signaling Technology, Inc., Danvers, MA | D21H3 |
| β-actin (rabbit monoclonal) | Cell Signaling Technology, Inc., Danvers, MA | 13E5 |
| Aggrecan (rabbit polyclonal) | MilliporeSigma, Bedford, MA | AB1031 |
| Chondroitin sulfate (mouse monoclonal) | Abcam, Cambridge, MA | ab34710 |
| Collagen II (rabbit polyclonal) | Fitzgerald Industries International, Acton, MA | 70R-CR008 |
| Collagen I (rabbit polyclonal) | Abcam, Cambridge, MA | ab34710 |
| Sonic Hedgehog (rabbit polyclonal) | Novus Biologicals, Centennial, CO | NBP2-22139 |
| Patched 1 (rat monoclonal) | R&D Systems, Inc., Minneapolis, MN | MAB41051 |
| Smoothened (rabbit polyclonal) | Abcam, Cambridge, MA | ab72130 |
| Gli1 (rabbit polyclonal) | Abcam, Cambridge, MA | ab151796 |
| IFT88 (rabbit polyclonal) | MilliporeSigma, Bedford, MA | ABC932 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Smoothened agonist (SAG) | Abcam, Cambridge, MA | Ab142160 |
| Cylcopamine (CyA) | LC Laboratories, Woburn, MA | C-8700 |
| Critical Commercial Assays | ||
| In situ Cell Death Detection Kit, TMR Red | MilliporeSigma, Bedford, MA | 12156792910 |
| Experimental Models: Cell Lines | ||
| HEK 293 T cells | ATCC, Manassas, VA | CRL-3216 |
| Experimental Models: Organisms/Strains | ||
| Nfat5+/Δ mice | Provided by H. Moo Kwan, Ulsan National Institute of Science and Technology | |
| Ift88f/f mice | Provided by Bradley Yoder, University of Alabama | |
| ShhcreERT2 mice | The Jackson Laboratory | 005623 |
| Sprague Dawley Rats | Charles River, Wilmington, MA | Code: 400 |
| RT-PCR Oligonucleotides | ||
| Nfat5 mouse genotyping WT (F): 5’-AAGGGCTTCTTCCAGAATGG-3’ | Integrated DNA Technologies, Inc. Skokie, IL | |
| Nfat5 mouse genotyping NFAT5+/Δ (F): 5’-GTGTTTCTCAGCCAAGTAAGGTC-3’ | Integrated DNA Technologies, Inc. Skokie, IL | |
| Nfat5 mouse genotyping (R): 5’-GGGCTATAGACATGCACCACCACACAG-3’ | Integrated DNA Technologies, Inc. Skokie, IL | |
| Ift88f/f mouse genotyping (common 5’ primer): GCCTCCTGTTTCTTGACAACAGTG | Integrated DNA Technologies, Inc. Skokie, IL | |
| Ift88f/f mouse genotyping (3’ flox and wild type primer): GGTCCTAACAAGTAAGCCCAGTGTT | Integrated DNA Technologies, Inc. Skokie, IL | |
| Ift88f/f mouse genotyping (3’ delta allele primer): CTGCACCAGCCATTTCCTCTAAGTCATGTA | Integrated DNA Technologies, Inc. Skokie, IL | |
| ShhcreERT2 mouse genotyping (transgene forward): 5’-TGGCTACCCGTGATATTGCT-3’ | Integrated DNA Technologies, Inc. Skokie, IL | |
| ShhcreERT2 mouse genotyping (transgene reverse): 5’-GAGCGGCGATACCGTAAAG-3’ | Integrated DNA Technologies, Inc. Skokie, IL | |
| ShhcreERT2 mouse genotyping (internal positive control forward): 5’-CACGTGGGCTCCAGCATT-3’ | Integrated DNA Technologies, Inc. Skokie, IL | |
| ShhcreERT2 mouse genotyping (internal positive control reverse): 5’-TCACCAGTCATTTCTGCCTTTG-3’ | Integrated DNA Technologies, Inc. Skokie, IL | |
| qRT-PCR Oligonucleotides | ||
| TonEBP (F) 5’-AAACGAAATCCAAAGCAGAGGCCG-3’ | Integrated DNA Technologies, Inc. Skokie, IL | |
| TonEBP (R) 5’-TTGCCTTTGTCCGTGGTAAGC-3’ | Integrated DNA Technologies, Inc. Skokie, IL | |
| Shh (F) 5’-AGCCTACAAGCAGTTTATCCC-3’ | Integrated DNA Technologies, Inc. Skokie, IL | |
| Shh (R) 5’-GTTCCTTAAATCGTTCGGAGTTTC-3’ | Integrated DNA Technologies, Inc. Skokie, IL | |
| Ptch1 (F) 5’-AGACAAGCCCATCGACATTAG-3’ | Integrated DNA Technologies, Inc. Skokie, IL | |
| Ptch1 (R) 5’-GCGGTCAGGTAGATGTAGAAAG-3’ | Integrated DNA Technologies, Inc. Skokie, IL | |
| Smo (F) 5’-TGAGTGGCATCTGCTTTGT-3’ | Integrated DNA Technologies, Inc. Skokie, IL | |
| Smo (R) 5’-GGAAGTAGCCTCCCATAAG-3’ | Integrated DNA Technologies, Inc. Skokie, IL | |
| Gli1 (F) 5’-ATGGATACTAGAGGGCTACAGG-3’ | Integrated DNA Technologies, Inc. Skokie, IL | |
| Gli1 (R) 5’-CCAGAGTGTCAGCAGAAGAAA-3’ | Integrated DNA Technologies, Inc. Skokie, IL | |
| Plasmids | ||
| shNFAT5 | MilliporeSigma, Bedford, MA | TRCN0000020019 |