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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: J Allergy Clin Immunol. 2019 Jul 11;144(5):1228–1241.e9. doi: 10.1016/j.jaci.2019.06.037

Figure 4.

Figure 4.

IL-13 induces 15LO1 and CCL26 expression in ALI cultured NECs and 15LO1 positively correlates with CCL26 expression in vitro. ALI cultured primary NECs were stimulated with IL-13 for 5 days. Cells were harvested for RT-PCR and Western blot analysis. Lower supernatants were harvested for CCL26 levels by ELISA. IL-13 increased (A) 15LO1 mRNA expression, (B) CCL26 mRNA expression and (C) 15LO1 mRNA correlates with CCL26 mRNA expression in vitro. (D) IL-13 induces intracellular 15LO1 and CCL26 protein in cell lysates. (E) IL-13 induced 15LO1 protein correlates with CCL26 protein in vitro. Abbreviations: ALI=air-liquid interface, NECs=nasal epithelial cells.