(A-D) H&E staining of midsagittal sections of the cerebella from Non-IR and IR Nes-mYapTaz cKOs and controls at P30. Scale bars, 500 μm. (E) Graph of the midline cerebellar areas of IR mice as a percentage of Non-IR animals of the same genotype. P = 0.3815. (F-l) IF detection of NeuN on midsagittal sections of the cerebella from Non-IR and IR animals at P30. Scale bar, 500 μm. (J,K) Graphs of the areas of the IGL (J) and IGL/cerebellum ratios (K) of IR mice as a percentage of Non-IR animals of the same genotype on midsaggital sections at P30. Nes-mYapTaz cKOs (Non-IR, n = 7; IR, n = 6) and controls (Non-IR, n = 4; IR, n = 7). (J) p = 0.1637; (K) p = 0.0383. Data are presented as mean ± S.D., and statistical analysis by unpaired t test. Each data point represents one animal, and is calculated using the average for each IR mouse divided by the mean of the Non-IR mice of the same genotype, X 100.