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. Author manuscript; available in PMC: 2020 Nov 7.
Published in final edited form as: Cell Stem Cell. 2019 Sep 5;25(5):682–696.e8. doi: 10.1016/j.stem.2019.08.003

Fig 1. Hematopoietic specific loss of Stag2, but not Stag1, results in altered stem cell function.

Fig 1.

A) Stag2 KO, but not Stag1 KO mice have increased LSK (Lin−Kit+Sca1+) hematopoietic stem cells (Log2FC=2.2; p<0.01) B) Whole bone marrow plated in cytokine-enriched methylcellulose. Stag2 KO marrow, but not WT and Stag1 KO, has increased self-renewal capacity and can serially replate (>7 platings). Competitive bone marrow transplantation of C) Stag2 WT (Red)/KO (Blue) or D) Stag1 WT (Green)/KO (Purple) bone marrow mixed 1:1 with Cd45.1 normal marrow. Stag2 KO shows increased chimerism in stem and progenitor populations in the bone marrow at 16 weeks. E) Kaplan-Meier curve shows Stag2−/y/Stag1−/− is lethal with a median survival of 0.7 weeks (p=0.01). Stag2−/y/Stag1−/+ has a lethal phenotype with a median survival of 27.7 weeks (p=0.05). F) Hematoxylin and eosin (H&E) staining of Stag2/Stag1 KO bone marrow reveals marked aplasia. G) Left: t-SNE projection of library-size normalized and log transformed data for inferred HSC subset (2025 cells). Each dot represents a single cell colored by genetic condition (Stag2 KO (shades of blue), Stag2 WT (shades of red). Middle: t-SNE projection colored by the second and third principal components most correlated with lymphoid priming and cell cycle, respectively. Right: Distribution of cells along lymphoid and cell cycle components; p-values for Mann-Whitney U test are shown.