Skip to main content
. Author manuscript; available in PMC: 2020 Nov 7.
Published in final edited form as: Cell Stem Cell. 2019 Sep 26;25(5):666–681.e5. doi: 10.1016/j.stem.2019.08.014

Figure 4: Temporally asymmetric microtubules in GSCs.

Figure 4:

3D reconstructed montage made from live cell imaging using a nanos-Gal4; UAS-α-tubulin-GFP line in GSCs (A) and quantified in (D, n= 22, Table S8), as well as in SGs (C) and quantified in (E, n= 24, Table S9). Metaphase was set as time 0, and other time points prior to metaphase were labeled as minus minutes accordingly. (A) In GSCs the mother centrosome is activated in mid G2 (− 250 min) prior to the daughter centrosome at G2/M transition (− 50 min). (B) Using Airyscan, more microtubules emanating from mother centrosome could be detected from mid- to late-G2 phase GSCs. (C) In SGs, both centrosomes are active at the G2/M transition (−35 min) simultaneously. (D-E) All ratios= Avg± SE, see Table S8. Magenta arrow: microtubules poke-in. Asterisk: hub. Scale bars: 2μm (A, C), 5μm (B).